Check Latest Sample Paper for Last Minute Revision

CBSE Class 12 Biotechnology Sample Paper 2024

1.Type II Restriction Enzymes are employed and not type I and type III, in Recombinant DNA technology because :

(a) They recognise a palindromic recognition sequence.

(b) They lyse specifically within the restriction site.

(c) They cleave 1 bp away from the 5’ end.

(d) They cleave both the DNA strands simultaneously.

2.A bull has trouble walking and getting up, He is nervous and violent. This condition is getting worse with time. The causative agent responsible for these symptoms could be :

(a) Bacillus anthracis

(b) Virion

(c) Mycobacterium bovis

(d) Prion

3.Turbidity measurement method for ascertaining the number of microbial cells in a liquid culture is not accurate. Choose the correct explanation.

(a) It measures both live and dead cells.

(b) It measures low amount of cells too.

(c) It is difficult and non sensitive technique.

(d) It destroys the sample solution.

4.To increase the efficiency of picking of insert by a vector, Dr. Kumar decided to make use of ___________________ in the genetic engineering protocol. Identify the tool.

(a) DNA Ligase

(b) DNA Phosphatase

(c) Alkaline Phosphatase

(d) DNA Polymerase

5.Example of Single Cell Protein

(a) Yeast

(b) Bacteria

(c) Algae

(d) All of these

6.The first human cell line established by George Gay in 1950s from cervix cancer is called ____________

(a) CHO cell line

(b) Cos-1 cell line

(c) HeLa cell line

(d) Fibroblast cell line

7.In 1603, Baricelli reported that a spectrum of diseases like jaundice, skin lesions etc can be treated by administration of :

(a) Curd

(b) Honey

(c) Milk

(d) Whey

8.An important plant secondary metabolite produced through tissue culture which has found use in antifertility drugs is _________________

(a) Berberine

(b) Quinine

(c) Shikonin

(d) Diosgenin

9.One of the important purpose of Functional Proteomics is :

(a) To obtain 3D structure of all the proteins

(b) To identify protein networks in nuclear pore complex

(c) To characterise all protein-protein interactions

(d) To identify disease specific proteins

10.The vector that was used in the first cloning experiment involving mammalian host cells was _________________

(a) Adenovirus

(b) Papillomavirus

(c) Retrovirus

(d) SV40 virus

11.Name the autosomal recessive disorder that follows mendelian inheritance and occurs due to deletion of 3 base pairs resulting in loss of codon 508 which codes for phenylalanine.

(a) Huntington disease

(b) Migraine

(c) Cystic fibrosis

(d) Alzheimer

12.Leuconostoc mesenteroides is used for the commercial production of:

(a) Dextran

(b) Ethanol

(c) Penicillin

(d) Amylases

Question No. 13 to 16 consist of two statements – Assertion (A) and Reason (R). Answer these questions selecting the appropriate option given below:

(a) Both A and R are true and R is the correct explanation of A.

(b) Both A and R are true and R is not the correct explanation of A.

(c) A is true but R is false.

(d) A is false but R is true.

13.Assertion – It is very difficult to produce hybrids in inter-generic crosses.

Reason – The abnormal development of endosperm leads to death of hybrid embryo.

Answer: (a)Both Assertion and Reason are true and reason is the correct explanation of the assertion.

14.Assertion– Humulin acts in 15 minutes whereas classical insulin takes 3 hours.

Reason –Hybridoma technology can facilitate the development of faster acting proteins like Humulin.

Answer: (c) Assertion is true but the reason is false.

15.Assertion – Animal cells in vitro divide till they fill the surface of the culture vessel and then stop growing.

Reason – Animal cells can be grown upto only limited generations.

Answer: (b) Both the Assertion and reason are true but reason is not the correct explanation of the assertion.

16.Assertion – The number of proteins easily outnumber the number of genes.

Reason – It is estimated that proteins can undergo approximately 200 different types of post transcriptional modifications.

Answer: (c) The assertion is true but the reason is false.

To check all questions of the paper, download PDF from the following link: